f gene造句

• The f gene from the resulting recombinant plasmid pcdna3-f was sequenced
  序列测定结果表明，克隆的f基因全长1668bp，编码553个氨基酸。
• Phylogenic tree from 29 strains were draw based on the ndv hn gene ( part segment ), and the result is similar to that of f gene
  根据29株ndvhn基因部分编码序列绘制ndv系统发育进化树，发现f及hn基因的分型结果大致相同。
• The main purpose of this study was to examine the immunogenicity of a dna vaccine against ndv using the attenuated s . typhimurium with dam and phop mutations as a vector for delivery of the ndv f gene
  本研究探讨了利用沙门氏菌dam基因和phop基因双突变株为载体传递ndvdna疫苗的免疫原性和可行性。
• All the isolates in the study of genotype vii could be recoganized by the new cutting site at nt 872 by re hinf i which is absent in the genetic supgroups viia, viib, and group vi . the amino acid sequences of the coding region of the f gene were deduced
  由核昔酸序列推导了f蛋白高变区1一125位氨基酸(aa)，通过分析比较发现了新的基因型和基因亚型与其他基因型毒株的差异还体现在aa位点的变化上。
• Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells . there was expression of the f protein as shown by indirect immunofluorescent assay . the expression began at 48h post-infection and increased thereafter, as indicated by elisa
  将真核表达质粒pcdna3-f高压电转化dam和phop基因双突变的减毒鼠伤寒沙门氏菌zj111株(zj111/pcdan3-f)，并直接转染vero细胞，分别提取细胞总dna和总rna，dig标记探针均可检测到阳性杂交信号。
• Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene, a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt-pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
  利用从国外引进的新城疫热稳定性天然弱毒b_(95)株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应(rt-pcr)扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t-a克隆技术将其克隆到pgem-t-easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、pcr鉴定和酶切分析筛选阳性克隆。
• Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene, a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt-pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
  利用从国外引进的新城疫热稳定性天然弱毒b_(95)株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应(rt-pcr)扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t-a克隆技术将其克隆到pgem-t-easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、pcr鉴定和酶切分析筛选阳性克隆。
• Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome-mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3-4 hours earlier
  puc18ha和sk质粒同时经hind、kpn酶切后连接得中间质粒skha；将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点，通过酶切鉴定获得了pfgs11ha；将含ndvf基因的质粒puc19f用hind、sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe/l启动子下获得中间质粒skf，再将质粒skf和puc18质粒先分别用ecor、xho酶切klenow补平，后再共同用sac酶切连接得puc18pelf，sal酶切回收pe/l-f基因盒插入到pfgs11ha的sal位点，通过酶切鉴定获得了pe/l-f与ps-ha同向的表达载体pfgs11haf。
• Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome-mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3-4 hours earlier
  puc18ha和sk质粒同时经hind、kpn酶切后连接得中间质粒skha；将质粒skha用bamhi酶切回收ha基因插入到插入载体pfgs11中的bamhi位点，通过酶切鉴定获得了pfgs11ha；将含ndvf基因的质粒puc19f用hind、sal酶切经klenow酶补平插入到经sma酶切后的skifn中pe/l启动子下获得中间质粒skf，再将质粒skf和puc18质粒先分别用ecor、xho酶切klenow补平，后再共同用sac酶切连接得puc18pelf，sal酶切回收pe/l-f基因盒插入到pfgs11ha的sal位点，通过酶切鉴定获得了pe/l-f与ps-ha同向的表达载体pfgs11haf。
• The n-terminal nucleotides 47-420 of the guangxi apmv-1 isolates of different poultry species origin were amplified and sequenced . the alignment and phylogenetic analysis of the nucleotide sequences and deduced amino acid sequences of f gene of the guangxi isolates and other reference strains obtained from genbank were done
  研究对鸡、鹅、鸽三种禽源apmv刁广西分离株f基因n与前段进行了rtpcr扩增及核茸酸序列测定，并用基因分析软件dnastar进行分析并与已发表的其它参考毒株进行比较。
• The specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis . the results indicated that the resuctant construct contained the gene of interest f . the positive cloned was sequenced by sanger's dideoxy sequencing method . the results demonstrated that the f gene included an open reading frame which was 1662bp in length and encoded a protein of 553 amino acid
  经核苷酸测序，该基因含一个1662bp的开放阅读框，编码553个氨基酸，与国外报导的新城疫各毒株f基因相比，核苷酸序列同源性为88.396.4，氨基酸同源性为92.196.4。
• We immunize the balb / c mice with pc4.0f and pc3.1f by paunch and muscle administration and immunize each with different content of 10 g, 50 g 100 g . the antibodies in serum against newscastle disease virus f gene protein are tested by elisa . the result showes that two kinds of dna vaccines can induce specific antibody response to encoded proteins in 15 weeks by im and ip, but antibodies response is little different
  免疫结果显示，采用2种免疫途径(肌肉注射，腹腔注射)，3个剂量(10g、50g和100g)免疫balbc小鼠，在被检测的15周内，肌肉注射和腹腔注射组均可产生抗体，抗体的产生有明显的剂量依赖性，50g只和100g只明显优于10g只，而100g只与50g只相似(肌肉注射)甚至稍低(腹腔注射)。
• Epitope is the mini-unit to induce immune response . identify the epitopes on ndv will laid solid foundation for development of the nd epitope-vaccine . f gene comparison of ndv isolates in china showed that ndv isolates in china are mainly belong to branches of group based on the phylogenetic tree made by the 1-374 nucleotide of f gene
  比较基因型各毒株之间核苷酸序列，发现各毒株之间同源率在91.7-99.7之间，在本实验室分离并保留的毒株中，gx-2-98与其他各毒株之间同源率多数是在95以上，具有良好的代表性。
• Epitope is the mini-unit to induce immune response . identify the epitopes on ndv will laid solid foundation for development of the nd epitope-vaccine . f gene comparison of ndv isolates in china showed that ndv isolates in china are mainly belong to branches of group based on the phylogenetic tree made by the 1-374 nucleotide of f gene
  比较基因型各毒株之间核苷酸序列，发现各毒株之间同源率在91.7-99.7之间，在本实验室分离并保留的毒株中，gx-2-98与其他各毒株之间同源率多数是在95以上，具有良好的代表性。
• Major difference of f gene lies in no . 1-no . 32 amino acid residues on n-terminal, which is the signal sequence of f protein and cut off short after the new peptide of f protein is combined with the membrane of vesicle . the sequence is not the composition of the f protein and the evolutronary force it bears is very small
  氨基酸的差异主要集中在n端1-32残基，该序列为f蛋白的信号序列，在f蛋白新生多肽与囊膜泡结合后不久被切下来，因此他不是功能性f蛋白的构成成分，所承受的进化强制就很小。
• 374-nt sequence analysis between nt 47-420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34-1682 were used to compare the 18 isolates for genetic analysis . a phylogenic tree was constructed based on the 374-nt-sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources
  通过dnastar软件对f基因47470nt间片段进行同源性分析比较并绘制了遗传进化树枝状结构发生图，结合3341672nt间三种限制性内切酶(re：hinf，bsto及rsa)位点的分布情况，确定了这些分离株的基因分类地位。